Therefore, the wavelength of maximum absorption by a substance is one of the characteristic properties of that material. Different substances absorb different wavelengths of light. The spectrum of pure bilirubin is known thus the molar absorbance is known. Because Beer's law states this, it means we can both calculate the concentration of a solution by using the absorbancies, or plot a graph of various concentrations, align them to their correct absorbencies, and use a colorimeter to find the concentration of an unknown solution The law states that:. Contents 1 Definition & Equation 2 Derivation of Law 3 Deviations to the law 4 Applications 5 Links 5.1 Related articles 5.2 External links 5.3 Bibliography . The law was first developed by Pierre Bouguer before 1729.
A spectrophotometer is an apparatus that measures the intensity, energy carried by the radiation per unit area per unit time, of the light entering a sample solution and the light going out of a sample solution. The absorbance is inversely proportional to the transmittence of the solution. Deviations to the law[ edit edit source]. A completely transparent substance will have It = I0 and its percent transmittance will be 100. It was later attributed to Johann Heinrich Lambert who cited Bouguers findings. Measurements are made at one specific wavelength almost unique for bilirubin and another measurement at a second wavelength so interferences or deviations can be eliminated or corrected. ..
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